Normal human urinary porphyrins

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Urinary Porphyrins in Disease

The structural chemistry of the blood and bile pigments has been developed by Hans Fischer and his school (l), but the physiological conditions which lead to the increased production of these pigments, especially of the porphyrins, are still uncertain. Although it is well known that in many diseases, notably those characterized by fever or liver injury (2), an increased excretion of porphyrins ...

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Urinary porphyrins and porphyrin precursors in normal pregnancy. Relationship to urinary total oestrogen excretion,.

The relationship between urinary excretion of porphyrins, porphyrin precursors and total oestrogens in normal pregnancy was investigated. Significant increases in total oestrogen, delta-aminolaevulinic acid (ALA) and coproporphyrin (COPRO) excretion were noted. However, no close correlation was found between total oestrogen excretion and urinary output of ALA and COPRO. The results suggest that...

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Liquid-chromatographic analysis for urinary porphyrins.

Urinary porphyrins are separated, according to number of carboxyl groups, in a system consisting of a mu-Bondapak C18 stationary phase and a mobile phase of methanol and aqueous sodium phosphate (pH 3.5) in a linear gradient. The specimen is prepared simply by adjusting the pH of a 5-mL sample to 2.0 and removing solids by centrifugation. The eluted porphyrins are measured fluorometrically. Nat...

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Urinary porphyrins in lead-treated rabbits.

-* acetate -> fatty acid. We express our gratitude to Dr R. Bentley and to Dr K. Bloch both of whom generously provided us with the samples of [1-14C]glucose. The photosynthetic experiments with tobacco leaves were carried out by Dr Helen Porter at the Botany Department of Imperial College of Science and Technology, whom we thank for her co-operation. The skilful technical assistance of Miss Ch...

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Liquid-chromatographic profiles of urinary porphyrins.

Information on changes in the urinary excretion pattern of porphyrins can be especially useful in the diagnosis of disorders of porphyrin metabolism. Most clinical laboratory procedures are designed for assay of uroporphyrin and coproporphyrin only, and in many cases even these are not cleanly separated. Hence, we developed a "high-performance" liquid-chromatographic procedure to separate and q...

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ژورنال

عنوان ژورنال: Biochemical Journal

سال: 1954

ISSN: 0306-3283

DOI: 10.1042/bj0580177